Coronavirus: Antibody test Kit and Nucleic Acid Test Kit
Brief Difference between Nucleic Acid Test kit and Antibody Test Kit.
Nucleic Acid Test Kit
What is Nucleic Acid Test (NAT) and how it works may be find in this article: What is Nucleic Acid Detection?. In this article, just compare the difference of Nucleic Acid Test and Antibody Test.
Nucleic Acid Test currently is the main diagnostic test method to check and confirm the suspected Coronavirus patients, because it has strong specificity and relatively high sensitivity by detecting the existence of the viral nucleic acid in our collected specimen directly.
The most popular method of Nucleic Acid test is Fluorescence PCR. Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make millions to billions of copies of a specific DNA sample allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.
Normally there are several steps for a Nucleic Acid Test: Sample processing, nucleic acid extraction, PCR detection, and etc. They are:
- Sample collection (nasopharyngeal swabs)
- nucleic acid extraction (nucleic acid extraction equipment)
- and detection of novel coronavirus;
some new extraction-free coronavirus nucleic acid detection reagents have been released. The average detection time takes 2-3 hours.
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Antibody Test Kit
Method of antibody test kit now includes Colloidal Gold and Magnetic Particle Chemiluminescence. Antibody Test will allow the determination of whether a person has ever been infected and will work regardless of whether the person developed symptoms. It is hoped that it can return results in 15 minutes by detecting both IgM and IgG antibodies.
Normally antibody test needs average 15min (Colloidal Gold) or 30-60min (Magnetic Particle Chemiluminescence) with full blood.
Antibody testing is to detect the level of antibodies in the human blood. In the early stages of disease infection, the body may not have produced antibodies, so it has a time window of detection. Especially for coronavirus.
Therefore, antibody detection can be used for auxiliary diagnosis of cases with negative nucleic acid tests, and can also be used for screening and screening of cases, but it cannot replace nucleic acid detection methods.
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